《植物生理学报》 2016, 52(8): 1248-1254

药用野生稻叶中花色苷合成基因的序列特点与表达差异

秦宗燕^*, 刘石娟^*, 包颖^**

曲阜师范大学生命科学学院, 山东曲阜273165

通信作者：秦宗燕；E-mail: baoyingus@126.com

摘　要：

为全面把握花色苷合成基因在非模式植物绿色组织中的构成和表达, 本研究利用转录组重测序方法, 对药用野生稻(Oryza officinalis)绿叶中出现的花色苷合成基因进行了分析, 结果共鉴定出8个相关基因(CHS、CHI、F3H、F3′H、F3′5′H、DFR、ANS和UFGT), 这些基因与栽培稻同源基因对之间在mRNA水平上的序列相似度达到了94%~96%。相对外类群的K_a/K_s (非同义替换率与同义替换率的比值)统计检验进一步表明这8个基因均处于严格的纯化选择之下。RPKM (每百万读段中每千个碱基长度的读段数)值分析表明, 8个基因的表达水平不同, 其中F3′5′H的相对表达量最高, CHI和CHS次之, F3′H最低。

关键词：药用野生稻; 叶转录组; 花色苷合成途径; 基因表达

收稿：2016-05-10   修定：2016-07-21

资助：国家自然科学基金(31570218)。 致谢 国际水稻研究所(International Rice Research Institute, IRRI)提供药用野生稻种子样品。

Sequence characteristic and expression analysis of genes related to anthocyanin biosynthesis in Oryza officinalis leaf transcriptome

QIN Zong-Yan^*, LIU Shi-Juan^*, BAO Ying^**

School of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China

Corresponding author: QIN Zong-Yan; E-mail: baoyingus@126.com

Abstract:

In order to completely understand member composition and expression of the genes about anthocyanin synthesis in green tissues of non-model plant, the leaf transcriptome of Oryza officinalis was re-sequenced, and the genes in the anthocyanin synthetic pathway were analyzed. A total of 8 genes (CHS, CHI, F3H, F3′H, F3′5′H, DFR, UFGT and ANS) were identified. These genes shared high-level sequence similarities (94%~96%) regarding to the mRNA with their homologous genes in cultivated rice, respectively. Moreover, the statistical tests of the ratio of non-synonymous substitutions rate to synonymous substitutions rate (K_a/K_s) relative to the outgroup show that the 8 genes were all influenced by the purified selection. In addition, reads per kilobase per million mapped reads (RPKM) comparison revealed a divergent expression among the 8 genes, among which F3′5′H had the highest expression level, following by CHI and CHS, and F3′H had the lowest expression level.

Key words: Oryza officinalis; leaf transcriptome; anthocyanin biosynthetic pathway; gene expression